|Detail of the metabolite profile
||Non-polar gas chromatography mass spectroscopy (GC-MS)
|Description for analysis method
||A portion of 0.5 mL organic extract was dried under N2 and redesolved with 40 mL CHCl3. The sample was directly analyzed with GC/MS with analysed using an Agilent 6890 GC coupled to a 5973 MSD scanning from m/z 50-650. Samples were injected at a 1:1 split ratio, and the inlet and transfer line were held at 280 ??C. Separation was achieved with a temperature programme of 40 ??C for 2 min, then ramped at 10 ??C/min to 310 ??C and held for 18 min, a 60 m DB-5MS column (J&W Scientific, 0.25 mm ID, 0.25 lm film thickness) and a constant flow of 1.0 ml/min.
|Species / Cultivar / Tissue
||Humulus lupulus / / Trichome
|Description for biological material
||Female cones were mashed in liquid nitrogen and glandular trichomes were separated from cones. The trichomes were shifted into a glass vial through a fine metal mesh. The samples were lyophilized prior to analysis.
||Don Sik Yang, Lloyd W. Sumner. Metabolic Profiling of trichomes from Potato Leafhopper susceptible and resistant alfalfa lines. (unpublished)
Plant Biology Division
The Samuel Roberts NOBLE Foundation, Inc.
Ardmore, OKLAHOMA, U.S.A - 73401.