Library Acc. LIBEST_026715
Name LIBEST_026715 Tobacco two-celled proembryo cDNA library
Organism Nicotiana tabacum
Stage after first zygote division
Description Vector: pTripEX2; Site_1: Sfi IA; Site_2: Sfi IB; Two-celled proembryo were manually collected from ovules. RNA was isolated and cDNA library was constructed by using the Clontech SMART cDNA library construction kit. The cDNAs were directionally ligated into pTripEX2.
# of EST/cDNA 2149
# of Unigene 1633; Of them, 325 singletons and 1308 TCs.
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Release Date Jul 14, 2011
Contact Haiping Xin Key Laboratory of the Ministry of Education for Plant Developmental Biology College of Life Science, Wuhan University Box3012, Hubei, 430072 China Tel: 862768756170 Fax: 862768756010 Email: Seq primer: pTripEX2 5' sequencing primer.
Citation Dynamic changes of transcript profiles after fertilization are associated with de novo transcription and maternal elimination in tobacco zygote, and mark the onset of the maternal-to-zygotic transition Zhao,J., Xin,H., Qu,L., Ning,J., Peng,X., Yan,T., Ma,L., Li,S. and Sun,M.X. Plant J. 65 (1), 131-145 (2011)