Library Acc. SRR027939
Name SRR027939 total trichomes from the leaves, 454 library
Organism Solanum pennellii
Tissue Total trichomes from the leaves
Description 454 run ID in NCBI: SRR027939, by the Michigan State University on 2009-10-06 10:20:43 SRX011590 Total trichomes from S. pennellii LA0716 Total RNA was extracted from total trichomes isolated from leaf tissue of Solanum pennellii LA0716 plants and used directly for cDNA synthesis using the Clontech SMART cDNA synthesis kit with slight modification to kit protocols. Reverse transcription of RNA was performed using a modified primer with sequence: 5'-TAGAGGCCGAGGCGGCCGACATGTTTTGTTTTTTTTTCTTTTTTTTTTVN-3'. Size selected SfiI digested cDNA was submitted for sequencing by the Michigan State University sequencing facility according to the standard Roche 454 GS-FLX protocol. Glandular trichomes play important roles in protecting plants from biotic attack by producing defensive compounds. We investigated the metabolic profiles and transcriptomes to characterize the differences between different glandular trichome types in several domesticated and wild Solanum species: Solanum lycopersicum (glandular trichome types 1, 6, and 7), Solanum habrochaites (types 1, 4, and 6), Solanum pennellii (types 4 and 6), Solanum arcanum (type 6), and Solanum pimpinellifolium (type 6). Substantial chemical differences in and between Solanum species and glandular trichome types are likely determined by the regulation of metabolism at several levels. Comparison of S. habrochaites type 1 and 4 glandular trichomes revealed few differences in chemical content or transcript abundance, leading to the conclusion that these two glandular trichome types are the same and differ perhaps only in stalk length. The observation that all of the other species examined here contain either type 1 or 4 trichomes (not both) supports the conclusion that these two trichome types are the same. Most differences in metabolites between type 1 and 4 glands on the one hand and type 6 glands on the other hand are quantitative but not qualitative. Several glandular trichome types express genes associated with photosynthesis and carbon fixation, indicating that some carbon destined for specialized metabolism is likely fixed within the trichome secretory cells. Finally, Solanum type 7 glandular trichomes do not appear to be involved in the biosynthesis and storage of specialized metabolites and thus likely serve another unknown function, perhaps as the site of the synthesis of protease inhibitors. Strategy: EST; Source: TRANSCRIPTOMIC; Selection: RANDOM; Layout: SINGLE; Construction protocol: Standard 454 library construction protocol; Platform: LS454; Instrument model: 454 GS FLX
# of EST/cDNA 344190
# of Unigene 158734; Of them, 153319 singletons and 5415 TCs.
Download (.zip) link
Release Date Oct 06, 2009
Contact the Michigan State University
Citation Plant Physiol. 2011 Jan;155(1):524-39. Epub 2010 Nov 19.Comparative functional genomic analysis of Solanum glandular trichome types.