Library Acc. AA_CAIT
Name CAIT Artemisia annua leaf
Organism Artemisia annua
Tissue leaf
Description Vector: p21_cDNA; Site_1: SalI; Site_2: NotI; The library was made from oligo(dT)-primed cDNA and cloned into the p21_cDNA vector. PolyA RNA was primed with an oligo(dT) primer (5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), ligated to a SalI adapter (5'-TCGACCCACGCGTCCG and 5'-CGGACGCGTGGG) and digested with NotI. cDNA was size selected from 1-2kb using 1.1% agarose gel electrophoresis then ligated into NotI and SalI-digested p21_cDNA vector. Library was created and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).
# of EST/cDNA 679
# of Unigene 395; Of them, 131 singletons and 264 TCs.
Download (.zip) link
Release Date Nov 07, 2007
Contact Lindquist,E.A., Richardson,P. DOE Joint Genome Institute 2800 Mitchell Drive, Walnut Creek, CA 94598, USA Tel: 925 296 5600 Fax: 925 296 5710 Email: cDNA Library Preparation: DOE Joint Genome Institute: cDNA Library Arrayed by: DOE Joint Genome Institute: DNA Sequencing: DOE Joint Genome Institute Clone Distribution: For inquiries regarding access to clones:Jay Keasling ( Plate: CAIT 0005 row: P column: 24.
Citation DOE Joint Genome Institute Artemisia annua EST project Newman,K.L., Keasling,J.D., Richardson,P., Lucas,S., Rokhsar,D., Wang,M. and Lindquist,E.A. Unpublished (2007)