Library Acc. LIBEST_025693
Name LIBEST_025693 AAGST
Organism Artemisia annua
Tissue glandular trichome
Stage 19-21 days after induction of flowering
Description Organ: flower bud; Vector: pDNR-LIB; Plants (seed source: Elixir Farm Botanicals, Brixey,MO, USA) were grown with 16 h/25 C days and 8 h/20 C nights to a height of approximately 1.2 m (about 3 months) then transferred to 12 h/25 C days and 12 h/20 C nights. Glandular trichomes were isolated by abrasion using a Bead Beater (Biospec) and filtration through nylon mesh. cDNA synthesis and library construction were carried out with a Creator SMART cDNA Library Construction Kit (Clontech) using Long-Distance PCR.
# of EST/cDNA 3690
# of Unigene 2276; Of them, 1473 singletons and 803 TCs.
Download (.zip) link
Release Date May 21, 2010
Contact Covello PS National Research Council Canada Plant Biotechnology Institute 110 Gymnasium Place, Saskatoon, SK, Canada S7N 0W9 Tel: 306 975 5269 Fax: 306 975 4839 Email:
Citation Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin Teoh,K.H., Polichuk,D.R., Reed,D.W., Nowak,G. and Covello,P.S. FEBS Lett. 580 (5), 1411-1416 (2006)