Library Acc. LIBEST_025694
Organism Artemisia annua
Tissue glandular trichome minus flower bud
Stage 19-21 days after induction of flowering
Description Organ: flower bud; Vector: pDNR-LIB; Plants (seed source: Elixir Farm Botanicals, Brixey,MO, USA) were grown with 16 h/25 C days and 8 h/20 C nights to a height of approximately 1.2 m (about 3 months) then transferred to 12 h/25 C days and 12 h/20 C nights. Glandular trichomes were isolated by abrasion using a Bead Beater (Biospec) and filtration through nylon mesh. A subtracted cDNA library was constructed with a PCR-Select cDNA Subtraction Kit (Clontech) using poly A+ RNA isolated from glandular trichomes (tester) and flower buds (driver).
# of EST/cDNA 3525
# of Unigene 1685; Of them, 1315 singletons and 370 TCs.
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Release Date May 21, 2010
Contact Covello PS National Research Council Canada Plant Biotechnology Institute 110 Gymnasium Place, Saskatoon, SK, Canada S7N 0W9 Tel: 306 975 5269 Fax: 306 975 4839 Email:
Citation Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin Teoh,K.H., Polichuk,D.R., Reed,D.W., Nowak,G. and Covello,P.S. FEBS Lett. 580 (5), 1411-1416 (2006)