Library Acc. SL_CALLUS
Name Solanum lycopersicum, callus,25-40 days old
Organism Solanum lycopersicum
Tissue callus
Stage 25-40 days old
Description Vector: pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; supplier: Boyce Thompson Institute; sequencing: The Institute for Genomic Research; cLEC - Cotyledons of seedlings 7-10 days post-germination were excised, cut at both ends and placed on MS medium with no selection. Mixed callus was harvested at 25 and 40 days and included undifferentiated masses. Tomato Callus EST Library
# of EST/cDNA 2408
# of Unigene 1890; Of them, 410 singletons and 1480 TCs.
Download (.zip) link
Release Date May 02, 2006
Contact CUGI Clemson University Genomics Institute Clemson University 100 Jordan Hall, Clemson, SC 29634, USA Email: This clone is available through the Clemson University Genomics Institute Seq primer: T3.
Citation Generation of ESTs from tomato callus tissue (2001) Alcala,J., Vrebalov,J., White,R., Vision,T., Karamycheva,S.A., Tsai,J., Utterback,T., Van Aken,S., Ronning,C.M., Fraser,C.M., Martin,G.B., Tanksley,S.D. and Giovannoni,J. Unpublished (2001)