Library Acc. HLUPJN1
Organism Humulus lupulus
Stage Trichomes isolated from mid to ripe stage hop cones
Description Vector: lambda-TriplEx2 (Clontech); Site_1: SfiIA; Site_2: SfiIB; The library HLUPJN1 was constructed from RNA isolated from lupulin glands from the variety 'Taurus'. Hop cones were collected near Mainburg, Germany in August 2002-2003. Lupulin glands were isolated by agitating dissected cones in liquid nitrogen followed by filtration to remove cone material. Total RNA was isolated using buffered phenol-chloroform-isoamyl alcohol followed by a clean-up with an RNeasy Plant Mini Kit (Qiagen); mRNA was purified using an Oligotex kit (Qiagen). The library was constructed using a SMART kit (Clontech). cDNAs were ligated into the phage vector lambda-TriplEx2 and packaged with MaxPlax Lambda packaging extract (Epicentre Technologies). For sequencing, cDNA inserts were PCR amplified from phage plaques using the vector primers 5'-CAAGCTCCGAGATCTGGACGAGC-3 and 5'-ATACGACTCACTATAGGGCGAATTGGCC-3'.
# of EST/cDNA 1872
# of Unigene 1153; Of them, 110 singletons and 1043 TCs.
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Release Date Sep 17, 2008
Contact Jonathan Page NRC Plant Biotechnology Institute 110 Gymnasium Place, Saskatoon, Saskatchewan, S7N 0W9, Canada Tel: 306-975-4187 Fax: 306-975-4839 Email:
Citation EST analysis of hop glandular trichomes identifies an O-methyltransferase that catalyzes the biosynthesis of xanthohumol Nagel,J., Culley,L., Lu,Y., Matthews,P.D., Stevens,J.F. and Page,J.E. Plant Cell 20 (1), 186-200 (2008)