Library Acc. LIBEST_024544
Name LIBEST_024544 NBREL2
Organism Nicotiana benthamiana
Description Organ: Root; Vector: pYL156; Site_1: BamH1; Site_2: EcoR1; The library NBREL2 was constructed using the suppression subtractive hybridization technique (Clontech PCR-select subtractive cDNA library kit) with RNA isolated from hydroponically-grown Nicotiana benthamiana plants as previously described (Liu & Page (2008) Plant Methods 4: 5). NBREL2 was created using mRNA from methyl jasmonate-treated roots as tester and untreated roots as driver. The subtracted cDNAs were amplified using primers containing BamH1 and EcoR1 sites, cloned directly into the TRV-RNA2 plasmid pYL156 (AF406991) and electroporated into Agrobacterium tumefaciens. cDNA inserts amplified from A. tumefaciens cultures were used as sequencing templates.
# of EST/cDNA 1424
# of Unigene 1057; Of them, 794 singletons and 263 TCs.
Download (.zip) link
Release Date Apr 26, 2009
Contact Jonathan Page NRC Plant Biotechnology Institute 110 Gymnasium Place, Saskatoon, Saskatchewan, S7N 0W9, Canada Tel: 306-975-4187 Fax: 306-975-4839 Email:
Citation Functional Genomics of Alkaloid Biosynthesis in Nicotiana benthamiana Li,R. and Page,J.E. Unpublished (2009)