Library Acc. MT_TRI
Name Medicago truncatula A17 glandular trichome
Organism Medicago truncatula
Tissue Isolated glandular trichomes
Stage Glands were isolated from young pre-flowering stems and leaves.
Description Vector: pDNR_lib (Clontech); Site_1: Sfi I A (GGCCATTACGGCC); Site_2: Sfi I B (GGCCGCCTCGGCC); Medicago truncatula A17 plants, were grown in pots containing 75% Metro Mix 350 soil and 25% sand. Glands were dislodged from tissues by vigorously vortexing tissues in an isotonic buffer. Glands were harvested by sieving out larger tissues and by differential centrifugation. RNA for library construction was isolated from pure gland preparations using the Qiagen RNA Easy kit. The Creator SMART cDNA Library Construction Kit from Clontech was used for library construction.
# of EST/cDNA 10377
# of Unigene 8005; Of them, 5827 singletons and 2178 TCs.
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Release Date Oct 09, 2007
Contact Zhao Xuechun Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73402, USA Tel: 580 224 6725 Fax: 580 224 6692 Email:
Citation Analysis of gene expression in glandular trichomes of Medicago truncatula Marks,M.D., Wenger,J., Wang,G., Zhao,X. and Dixon,R.A. Unpublished (2007)