Library Acc. AA_GTRI4
Name CATF Artemisia annua, Tanzanian, from Sandeman Seeds, 1-2kb
Organism Artemisia annua
Tissue glandular trichomes with 10-20% palisade cells and hair trichomes
Stage Transition to flowering
Description Vector: pDNR-LIB; Site_1: SfiI A; Site_2: SfiI B; The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected from 1-2kb using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library was created by the Keasling Research Group, University of California, Berkeley, and sequencing done by the DOE Joint Genome Institute (Walnut Creek, CA).
# of EST/cDNA 11366
# of Unigene 8185; Of them, 6477 singletons and 1708 TCs.
Download (.zip) link
Release Date Nov 09, 2007
Contact Lindquist,E.A., Richardson,P. DOE Joint Genome Institute 2800 Mitchell Drive, Walnut Creek, CA 94598, USA Tel: 925 296 5600 Fax: 925 296 5710 Email: Tissue Procurement: Keasling Research Group, UC Berkeley cDNA Library Preparation: Keasling Research Group, UC Berkeley cDNA Library Arrayed by: DOE Joint Genome Institute: DNA Sequencing: DOE Joint Genome Institute: Clone Distribution: For inquiries regarding access to clones:Jay Keasling ( Plate: CATF 0101 row: G column: 5.
Citation DOE Joint Genome Institute Artemisia annua EST project Newman,K.L., Keasling,J.D., Richardson,P., Lucas,S., Rokhsar,D., Wang,M. and Lindquist,E.A. Unpublished (2007)