||Nicotiana benthamiana trichomes
||Isolated stem trichomes
||Apical stem internodes of 5-week old plants.
||Vector: pDNR_lib (Clontech); Site_1: Sfi I A (GGCCATTACGGCC); Site_2: Sfi I B (GGCCGCCTCGGCC); Nicotiana benthamiana plants were grown on compost in the greenhouse with supplemental lighting (4 h in winter) providing a minimum illumination period of 14 h. Trichomes were harvested from stems of 5 week old N. benthamiana plants taken from the top half of the shoot. The stems were placed in 50 ml disposable falcon tubes and trichomes were abraded by vortexing for 20 s after freezing in liquid nitrogen. RNA was isolated using the Qiagen RNeasyMini kit. A cDNA EST library was generated following manufacturers instructions using the Creator SMART cDNA library construction kit (BD Biosciences) starting from total RNA and using the pDNR-LIB vector.
|# of EST/cDNA
|# of Unigene
Of them, 3747 singletons and 285 TCs.
||Dec 11, 2008
||NAME: Zhao X
FAX: 580 224 6692
TEL: 580 224 6725
LAB: Plant Biology Division
INST: The Samuel Roberts Noble Foundation
ADDR: 2510 Sam Noble Parkway, Ardmore, OK 73402, USA
||Transcriptomic and reverse genetic analyses of branched chain fatty acid and acyl sugar production in Solanum pennellii and Nicotiana benthamiana
Slocombe,S.P., Schauvinhold,I., McQuinn,R.P., Besser,K., Welsby,N.A., Harper,A., Aziz,N., Li,Y., Larson,T.R., Giovannoni,J., Dixon,R.A. and Broun,P.
Plant Physiol. 148 (4), 1830-1846 (2008)