||Solanum lycopersicum trichomes
||Isolated leaf trichomes
||Apical internodes of 5-week old plants.
||Vector: pDNR_lib (Clontech); Site_1: Sfi I A (GGCCATTACGGCC); Site_2: Sfi I B (GGCCGCCTCGGCC); Solanum lycopersicum plants were grown on compost in the greenhouse with supplemental lighting (4 h in winter) providing a minimum illumination period of 14 h. Trichomes were harvested from the most apical leaves of 5 week old Solanum lycopersicum plants. Trichomes were harvested by brushing leaves frozen using liquid nitrogen and separated from contaminating leaf fragments by passing the frozen material through a sieve. RNA was isolated from the trichomes using the Qiagen RNeasyMini kit. A cDNA EST library was generated following manufacturers instructions using the Creator SMART cDNA library construction kit (BD Biosciences) starting from total RNA and using the pDNR-LIB vector.
|# of EST/cDNA
|# of Unigene
Of them, 5588 singletons and 1391 TCs.
||Jul 03, 2007
||NAME: Zhao X
FAX: 580 224 6692
TEL: 580 224 6725
LAB: Plant Biology Division
INST: The Samuel Roberts Noble Foundation
ADDR: 2510 Sam Noble Parkway, Ardmore, OK 73402, USA
||Diverged regulation of terpenoid metabolism in the trichomes of a wild and cultivated tomato species
Besser,K., Harper,A., Welsby,N.A., Schauvinhold,I., Slocombe,S.P., Li,Y., Dixon,R.A. and Broun,P.