Library Acc. MtBA
Name Medicago truncatula, root tips after 3 days of N-starvation
Organism Medicago truncatula
Tissue root tips
Stage harvested after 3 days of N-starvation
Description Vector: pBluescript pSK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in an aeroponic chamber for 14 days on nitrogen-rich medium followed by 3 days on N-free medium. RNA was extracted from root tips (1-3 cm). cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into Uni-zapXR vector from Stratagene and packaged using Gigapack Gold packaging extracts. Plasmids containing cDNA inserts were mass-excised from phage stocks using ExAssit helper phage and propagated in SOLR cells. Clone ordering and sequencing was performed by the Centre National de Sequencage (Genoscope, Evry, France).
# of EST/cDNA 7932
# of Unigene 3709; Of them, 658 singletons and 3051 TCs.
Download (.zip) link
Release Date Aug 03, 2000
Contact Genoscope Genoscope - Centre National de Sequencage BP 191 91006 EVRY cedex - FRANCE Email:, Web : Contact : Pascal Gamas and Etienne-Pascal Journet, Laboratoire de Biologie Moleculaire des Relations Plantes-Microorganismes, CNRS-INRA, BP 27 31326 Castanet-Tolosan Cedex, France (Email : Website :
Citation Medicago truncatula ESTs from nitrogen-starved roots Journet,E.P., Crespeau,H., van-Tuinen,D., Gouzy,J., Jaillon,O., Niebel,A., Carreau,V., Chatagnier,O., Kahn,D., Gianinazzi-Pearson,V. and Gamas,P. Unpublished (2000)