Library Acc. MT_SROOT_KV1
Name Medicago truncatula, Seedling roots, inoculation with S. meliloti
Organism Medicago truncatula
Tissue Seedling roots
Stage 24 hours post-inoculation with Sinorhizobium meliloti
Description Vector: pBluescript SK -; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.
# of EST/cDNA 2840
# of Unigene 1962; Of them, 349 singletons and 1613 TCs.
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Release Date Oct 06, 2000
Contact VandenBosch K Department of Plant Biology University of Minnesota 220 BioSci Center, 1445 Gortner Ave, St. Paul, MN 55108, USA Tel: 612 624 2755 Fax: 612 625 1738 Email: Texas A&M University name: T268675e TIGR sequence name: MTIBJ95TK More information is available at: Seq primer: SKmod (CTA gAA CTA gtg gAT CC).
Citation ESTs from roots of Medicago truncatula 24 hours after inoculation with Sinorhizobium meliloti VandenBosch,K., Endre,G., Hur,J., Moore,J., Beremand,P., Ellis,L., Town,C.D., Bowman,C.L., Craven,M.B., Hansen,T.S., Holt,I.E. and Fraser,C.M. Unpublished (1999)