Library Acc. MT_VILEAF
Name Medicago truncatula,AMV-virus-infected leaves
Organism Medicago truncatula
Tissue Virus-infected leaves
Stage AMV infected leaves were harvested at 14 & 17dpi
Description Vector: Lambda Zap; Medicago truncatula leaves were infected with alfalfa mosaic virus (AMV). AMV infected leaves were harvested at 14 & 17dpi. Equal amounts of total RNA were isolated and pooled for mRNA purification. RNA for cDNA library construction was isolated from the pooled tissues. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.
# of EST/cDNA 7086
# of Unigene 2236; Of them, 185 singletons and 2051 TCs.
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Release Date Jan 11, 2005
Contact May GD Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73402, USA Tel: 580 224 6650 Fax: 580 224 6692 Email:
Citation Medicago truncatula Alfalfa Mosaic Virus- (AMV) Infected Leaves Expressed Sequence Tags from the Samuel Roberts Noble Foundation - Center for Medicago Genomics Research Liu,J., Scott,A.D., May,G.D. and Nelson,R.S. Unpublished (2005)