Library Acc. MT_SIRRA
Name Medicago truncatula, seedlings
Organism Medicago truncatula
Tissue seedlings
Stage seedling
Description Vector: Lambda Zap; Seedlings were exposed either to 100 gy gamma or 0.5, 1, 5, or 10 kJ/m2 UV irradiation. Gamma-irradiated samples were harvested at 6, 12, 24 and 48 hours after treatment. UV-irradiated samples were harvested 24 hours post-treatment. cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each sample. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.
# of EST/cDNA 5992
# of Unigene 2491; Of them, 664 singletons and 1827 TCs.
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Release Date Apr 24, 2002
Contact May GD Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73402, USA Tel: 580 224 6650 Fax: 580 224 6692 Email: Insert Length: 471 Std Error: 0.00 Plate: 108 row: H column: 08 Seq primer: TCACACAGGAAACAGCTATGAC.
Citation Expressed Sequence Tags from the Samuel Roberts Noble Foundation Medicago truncatula irradiated library Torres-Jerez,I., Scott,A.D., Harris,A.R., Gonzales,R.A., Bell,C.J., Flores,H.R., Inman,J.T., Weller,J.W. and May,G.D. Unpublished (2001)