Library Acc. GLSD
Name Medicago truncatula, Immature seeds
Organism Medicago truncatula
Tissue Immature seeds
Stage 25 to 35 days after pollination
Description Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; Immature seeds, collected from pods ranging in age from 25 to 35 days after pollination, were harvested from greenhouse-grown plants. Seed were removed and separated from pod walls and immediately frozen in liquid nitrogen. Seeds throughout the age range were pooled for mRNA extraction. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.
# of EST/cDNA 4864
# of Unigene 1545; Of them, 246 singletons and 1299 TCs.
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Release Date Jan 06, 2003
Contact Grusak, M.A. USDA/ARS Children's Nutrition Research Center Baylor College of Medicine 1100 Bates Street, Houston, TX 77030-2600, USA Tel: 713 798 7044 Fax: 713 798 7078 Email: TIGR sequence name: MTRCH96TK More information is available at: Seq primer: SKmod (CTA gAA CTA gtg gAT CC).
Citation ESTs from late stage developing seeds of Medicago truncatula Grusak,M.A., Samac,D., Town,C.D., Van Aken,S., Utterback,T., Cheung,F. and Fraser,C.M. Unpublished (2002)