Library Acc. MT_SROOT_KV0
Name Medicago truncatula, Seedling roots
Organism Medicago truncatula
Tissue Seedling roots
Stage Immediately prior to inoculation with Sinorhizobium meliloti (0 hour)
Description Vector: pBluescript SK -; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.
# of EST/cDNA 2752
# of Unigene 2031; Of them, 298 singletons and 1733 TCs.
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Release Date Apr 16, 2001
Contact VandenBosch K Department of Plant Biology University of Minnesota 220 BioSci Center, 1445 Gortner Ave, St. Paul, MN 55108, USA Tel: 612 624 2755 Fax: 612 625 1738 Email: Texas A&M University name:T266713e TIGR sequence name: MTGAL95TK More information is available at: Seq primer: SKmod (CTA gAA CTA gtg gAT CC).
Citation ESTs from uninoculated seedling roots of Medicago truncatula, 2001 VandenBosch,K., Endre,G., Hur,J., Moore,J., Beremand,P., Town,C.D., Van Aken,S., Utterback,T., Cho,J. and Fraser,C.M. Unpublished (2001)