Library Acc. MT_SROOT_KV2
Name Medicago truncatula, Seedling roots, inoculation with S. meliloti
Organism Medicago truncatula
Tissue Seedling roots
Stage 2 days post-inoculation with Sinorhizobium meliloti
Description Vector: pBluescript SK -; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in SOLR cells.
# of EST/cDNA 3330
# of Unigene 2283; Of them, 391 singletons and 1892 TCs.
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Release Date Mar 04, 2002
Contact VandenBosch K Department of Plant Biology University of Minnesota 220 BioSci Center, 1445 Gortner Ave, St. Paul, MN 55108, USA Tel: 612 624 2755 Fax: 612 625 1738 Email: TIGR sequence name: MTACK52TK More information is available at: Seq primer: SKmod (CTA gAA CTA gtg gAT CC).
Citation ESTs from roots of Medicago truncatula 48 hr after inoculation with Sinorhizobium meliloti VandenBosch,K., Endre,G., Hur,J., Beremand,P., Town,C.D., Van Aken,S., Utterback,T., Cheung,F., Tsai,J. and Fraser,C.M. Unpublished (2002)