Library Acc. MT_GPOD
Name Medicago truncatula, immature pod walls
Organism Medicago truncatula
Tissue immature pod walls
Stage Immature pods, ranging in age from 15 to 30 days after pollination
Description Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; Immature pods, ranging in age from 15 to 30 days after pollination, were collected from greenhouse-grown plants. At harvest, seeds were removed from pods and isolated pod walls were collected and immediately frozen in liquid nitrogen. Pod walls were pooled for mRNA extraction. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propogated in XLOLR cells.
# of EST/cDNA 3620
# of Unigene 2243; Of them, 299 singletons and 1944 TCs.
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Release Date Jan 21, 2003
Contact Grusak, M.A. USDA/ARS Children's Nutrition Research Center Baylor College of Medicine 1100 Bates Street, Houston, TX 77030-2600, USA Tel: 713 798 7044 Fax: 713 798 7078 Email: TIGR sequence name: MTOBO96TK More information is available at: Seq primer: SKmod (CTA gAA CTA gtg gAT CC).
Citation More ESTs from developing reproductive tissues of Medicago truncatula Grusak,M.A., Samac,D.A., Town,C.D., Van Aken,S., Utterback,T., Cheung,F. and Fraser,C.M. Unpublished (2002)