Library Acc. MT_LEAF_PHOMA
Name Medicago truncatula, leaf,Pathogen-induced
Organism Medicago truncatula
Tissue leaf
Stage Pathogen-induced, young trifoliate
Description Vector: pBluescript SK(-); Young trifoliate leaves of Medicago truncatula were excised and dip-inoculated in a spore suspension of Phoma medicaginis, and incubated in humid dishes. Pools of leaves were harvested at 0, 15, and 30 minutes and 1, 2, 3, 6, 14, 24, 48, 72, and 96, hours, and used to prepare total RNA. cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each sample. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.
# of EST/cDNA 3216
# of Unigene 2220; Of them, 325 singletons and 1895 TCs.
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Release Date Apr 26, 2002
Contact Paiva NL Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73402, USA Tel: 580 221 7317 Fax: 580 221 7380 Email: Insert Length: 152 Std Error: 0.00 Plate: 020 row: E column: 09 Seq primer: TCACACAGGAAACAGCTATGAC.
Citation Expressed Sequence Tags from the Samuel Roberts Noble Foundation Medicago truncatula Phoma-infected library Watson,B.S., Shin,H.-S., Lopez-Meyer,M., Scott,A.D., Harris,A.R., Gonzales,R.A., Bell,C.J., Inman,J.T., Waugh,M.E., Sullivan,J.P., May,G.D. and Paiva,N.L. Unpublished (2002)