Library Acc. MT_DFLOWER
Name Medicago truncatula, Developing flowers
Organism Medicago truncatula
Tissue Developing flowers
Stage Developmentally pooled. Contains a mixture of very young, developing, fully-opened flowers and flowers in early transition into pods.
Description Vector: Lambda Zap; cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from very young, developing, fully-opened flowers and flowers transitioning into pods. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.
# of EST/cDNA 6701
# of Unigene 4239; Of them, 781 singletons and 3458 TCs.
Download (.zip) link
Release Date Apr 24, 2002
Contact May GD Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73402, USA Tel: 580 224 6650 Fax: 580 224 6692 Email: Insert Length: 631 Std Error: 0.00 Plate: 080 row: A column: 12 Seq primer: TCACACAGGAAACAGCTATGAC.
Citation Expressed Sequence Tags from the Samuel Roberts Noble Foundation Medicago truncatula flower library Torres-Jerez,I., Scott,A.D., Harris,A.R., Gonzales,R.A., Bell,C.J., Flores,H.R., Inman,J.T., Weller,J.W. and May,G.D. Unpublished (2001)