Library Acc. MT_SEEDROOT_KV3
Name Medicago truncatula, Seedling roots
Organism Medicago truncatula
Tissue Seedling roots
Stage 3 days post-inoculation with Sinorhizobium meliloti
Description Vector: pBluescript SK -; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.
# of EST/cDNA 5913
# of Unigene 4018; Of them, 1004 singletons and 3014 TCs.
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Release Date Apr 24, 2003
Contact VandenBosch K Department of Plant Biology University of Minnesota 220 BioSci Center, 1445 Gortner Ave, St. Paul, MN 55108, USA Tel: 612 624 2755 Fax: 612 625 1738 Email: TIGR sequence name: MTEDJ96TK More information is available at: Seq primer: SKmod (CTA gAA CTA gtg gAT CC).
Citation ESTs from roots of Medicago truncatula 72 h after Rhizobium inoculation, 2001 VandenBosch,K., Endre,G., Hur,J., Beremand,P., Town,C.D., Van Aken,S., Utterback,T., Cho,J. and Fraser,C.M. Unpublished (2001)