Library Acc. MHRP-root
Name Medicago truncatula, roots,phosphate-starved
Organism Medicago truncatula
Tissue roots
Stage phosphate-starved
Description Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; At the trifoliate stage, M. truncatula plants were transplanted to phosphate-free sand and grown for a further 30 days. During this period, they were fertilized twice weekly with 1/2 Hoaglands solutions containing 20uM potassium phosphate. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.
# of EST/cDNA 2656
# of Unigene 1799; Of them, 297 singletons and 1502 TCs.
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Release Date Apr 12, 2001
Contact Harrison M.J. Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73401 Tel: 580-223-5810 Fax: 580-221-7380 Email: The Samuel Roberts Noble Foundation: N387937e TIGR sequence name: MTHBJ96TK More information is available at: Seq primer: SKmod (CTA gAA CTA gtg gAT CC).
Citation ESTs from phosphate-starved roots of Medicago truncatula, 2001 Harrison,M.J., Liu,J., Town,C.D., Van Aken,S., Utterback,T., Cho,J. and Fraser,C.M. Unpublished (2001)