Name Medicago truncatula, flowers, early seeds, late seeds and stems(MTY)
Organism Medicago truncatula
Tissue flowers, early seeds, late seeds and stems.
Description Vector: pDNR-LIB (Clontech); Site_1: SfiIA; Site_2: SfiIB; Total RNA isolated using TRIzol reagent. No DNaseI digestion was performed. Total RNAs were pooled together. Double strand cDNA were synthesized from pooled RNA using SMART technology (Clontech). The prepared cDNA was normalized by cDNA denaturation/reassociation, treatment by duplex-specific nuclease(DSN) and amplification of normalized fraction by PCR. The normalized cDNA was then digested with SfiI, size-fractioned, directionally ligated into pDNR-LIB (Clontech) and electroprated into GC10 competent cells (Gene Choice). Clones were sequenced from the 5' end only. RNA provided by Greg May.
# of EST/cDNA 4420
# of Unigene 3455; Of them, 448 singletons and 3007 TCs.
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Release Date Dec 15, 2005
Contact Cheung F Plant Genomics The Institute for Genomic Reserach 9712 Medical Center Dr., Rockville, MD 20850, USA Tel: 301 795 7829 Fax: 301 838 0208 Email: TIGR sequence name: MTYBI96TF Seq primer: TGT AAA ACG ACG GCC AGT.
Citation Construction and analysis of normalized cDNA library from flowers, seeds and stems of Medicago truncatula Cheung,F., Moskal,W., Cooper,J.D., Xiao,Y., May,G.D. and Town,C.D. Unpublished (2005)