Library Acc. MT_Shoots
Name Medicago truncatula, Aphid-infected shoots
Organism Medicago truncatula
Tissue Aphid-infected shoots
Stage harvested after 12, 24, 48, 72 and 96 hours of infestation
Description Vector: Lambda Zap; Medicago truncatula plants were infested with 300 spotted-aphids each. Tissue samples were collected by harvesting the entire shoots from 13 plants after 12, 24, 48, 72 and 96 hours of infestation. Total RNA was extracted from each sampling time and equal amounts of total RNA were pooled together for mRNA purification for cDNA library construction. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.
# of EST/cDNA 4943
# of Unigene 3123; Of them, 515 singletons and 2608 TCs.
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Release Date Jan 11, 2005
Contact May GD Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73402, USA Tel: 580 224 6650 Fax: 580 224 6692 Email:
Citation Medicago truncatula Aphid-Infected Shoot Expressed Sequence Tags from the Samuel Roberts Noble Foundation - Center for Medicago Genomics Research Zhang,Y., Chekhouskiy,K., Scott,A.D., May,G.D. and Mian,M.A.R. Unpublished (2005)