Library Acc. MT_GSEED
Name Medicago truncatula Germinating Seed
Organism Medicago truncatula
Tissue germinating seeds
Stage 0, 1, 2 and 3 days after acid treatment.
Description Vector: Lambda Zap; M. truncatula seeds were acid treated and placed on wet filter papers in petri dishes. Seeds were harvested at 0, 1, 2 and 3 days after acid treatment. cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each time point. The cDNA was directionally ligated into the Uni-Zap XR vector (Stratagene) and packaged using the Gigapack III Gold packaging extracts. Phagemids containing cDNA inserts were in vivo excised from the recombinant Uni-ZAP XR vector using ExAssist helper phage and the E. coli strain XL1-Blue MRF' (Stratagene). Excised plasmids were plated using SOLR cells.
# of EST/cDNA 7440
# of Unigene 3909; Of them, 863 singletons and 3046 TCs.
Download (.zip) link
Release Date Jan 12, 2005
Contact May GD Plant Biology Division The Samuel Roberts Noble Foundation 2510 Sam Noble Parkway, Ardmore, OK 73402, USA Tel: 580 224 6650 Fax: 580 224 6692 Email:
Citation Medicago truncatula Germinating Seed Expressed Sequence Tags from the Samuel Roberts Noble Foundation - Center for Medicago Genomics Research Torres-Jerez,I., Scott,A.D. and May,G.D. Unpublished (2005)