Library Acc. MtBB_NOD
Name Medicago truncatula, symbiotic root nodules
Organism Medicago truncatula
Tissue symbiotic root nodules
Stage harvested 4 days post inoculation with Sinorhizobium meliloti
Description Vector: pBluescript pSK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in an aeroponic chamber on nitrogen-rich medium for 21 days. Three days before inoculation with Sinorhizobium meliloti, the medium was replaced by N-free medium. Root nodules (+ short adjacent root segments) were harvested 4 days post inoculation. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into Uni-zap XR vector from Stratagene and packaged using Gigapack Gold packaging extracts. Plasmids containing cDNA inserts were mass-excised from phage stocks using ExAssit helper phage and propagated in SOLR cells. Clone ordering and sequencing was performed by the Centre National de Sequencage (Genoscope, Evry, France).
# of EST/cDNA 7807
# of Unigene 3213; Of them, 615 singletons and 2598 TCs.
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Release Date Aug 03, 2000
Contact Genoscope Genoscope - Centre National de Sequencage BP 191 91006 EVRY cedex - FRANCE Email:, Web : Contact : Pascal Gamas and Etienne-Pascal Journet, Laboratoire de Biologie Moleculaire des Relations Plantes-Microorganismes, CNRS-INRA, BP 27 31326 Castanet-Tolosan Cedex, France (Email : Website :
Citation Medicago truncatula ESTs from Sinorhizobium meliloti-induced root nodules Journet,E.P., Crespeau,H., van-Tuinen,D., Gouzy,J., Jaillon,O., Niebel,A., Carreau,V., Chatagnier,O., Kahn,D., Gianinazzi-Pearson,V. and Gamas,P. Unpublished (2000)