Library Acc. MtBC_GLOMUS
Name Medicago truncatula, noculated with Glomus intraradices
Organism Medicago truncatula
Tissue arbuscular mycorrhiza
Stage harvested 3 weeks post inoculation with Glomus intraradices
Description Vector: pBluescript pSK; Site_1: EcoRI; Site_2: XhoI; M. truncatula sterilised seeds were germinated for 72h at 25 C, before transplanting into a 1/3 Epoisses soil : 2/3 calcined Terragreen mix in the presence of onion root fragments colonized by the arbuscular mycorrhizal fungus Glomus intraradices (Schenck & Smith, isolate LPA8). The plants were watered every day and twice a week with a modified nutrient Long Ashton solution without phosphate but with a high level of nitrate. After 3 weeks RNA was extracted from whole root systems. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into Uni-zap XR vector from Stratagene and packaged using Gigapack Gold packaging extracts. Plasmids containing cDNA inserts were mass-excised from phage stocks using ExAssit helper phage and propagated in SOLR cells. Clone ordering and sequencing was performed by the Centre National de Sequencage (Genoscope, Evry, France). Note : EST may be of fungal origin.
# of EST/cDNA 8596
# of Unigene 4000; Of them, 1026 singletons and 2974 TCs.
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Release Date Aug 03, 2000
Contact Genoscope Genoscope - Centre National de Sequencage BP 191 91006 EVRY cedex - FRANCE Email:, Web : Contact : Pascal Gamas and Etienne-Pascal Journet, Laboratoire de Biologie Moleculaire des Relations Plantes-Microorganismes, CNRS-INRA, BP 27 31326 Castanet-Tolosan Cedex, France (Email : Website :
Citation Medicago truncatula ESTs from endomycorrhizal roots Journet,E.P., Crespeau,H., van-Tuinen,D., Gouzy,J., Jaillon,O., Niebel,A., Carreau,V., Chatagnier,O., Kahn,D., Gianinazzi-Pearson,V. and Gamas,P. Unpublished (2000)