Name Medicago truncatula mixed tissues
Organism Medicago truncatula
Tissue mixed tissues
Stage various stages
Description Vector: pBluescript SK-; Site_1: EcoRI; Site_2: XhoI; cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-assist helper phage and propagated in XLOLR cells.
# of EST/cDNA 6743
# of Unigene 4979; Of them, 433 singletons and 4546 TCs.
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Release Date Jul 21, 2003
Contact VandenBosch K Department of Plant Biology University of Minnesota 220 BioSci Center, 1445 Gortner Ave, St. Paul, MN 55108, USA Tel: 612 624 2755 Fax: 612 625 1738 Email: TIGR sequence name: MTUSZ96TK Alias Clone name:pGVSN-15M9 More information is available at: Seq primer: SKmod (CTA gAA CTA gtg gAT CC).
Citation The Medicago truncatula 6K unigene set: cDNA clones selected and re-arrayed from various libraries VandenBosch,K., Endre,G., Silverstein,K, Town,C.D., Van Aken,S., Utterback,T., Cheung,F. and Fraser,C.M. Unpublished (2002)